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| 日本臭菘体细胞胚胎发生及植株再生体系的建立 | |
| 引用本文： | 姜云天,陈艳秋,朱俊义,李玉梅,曲柏宏.日本臭菘体细胞胚胎发生及植株再生体系的建立[J].核农学报,2009,23(1):85-89. |
| 作者姓名： | 姜云天 陈艳秋 朱俊义 李玉梅 曲柏宏 |
| 作者单位： | 1. 通化师范学院生物系,吉林,通化,134002 2. 延边大学农学院园艺系,吉林,龙井,133400 3. 吉林师范大学,吉林,四平,136000 |
| 基金项目： | 通化师范学院自然科学基金 |
| 摘 要： | 以日本臭菘幼叶为外植体，应用均匀设计法筛选其最适合的胚性愈伤组织及胚性细胞复合体诱导、体细胞胚胎发育及植株再生的培养基配方，建立日本臭菘体细胞胚胎发生体系。结果表明，LS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L+IAA 0.1 mg/L适宜日本臭菘幼叶胚性愈伤组织及胚性细胞复合体诱导，诱导率为98%；LS+6-BA 0.50 mg/L+NAA 0.10 mg/L+IAA 0.10 mg/L最适宜体胚发育及植株再生，体胚萌发率为100%，萌发的体胚在发育培养基上继续培养42 d后全部发育成完整植株。对不同阶段培养材料的形态结构及超微结构的观察证明，成功建立了日本臭菘体细胞发生及植株再生体系。 |
| 关 键 词： | 日本臭菘 均匀设计 胚性细胞复合体 体细胞胚 植株再生 |
Establishment of Somatic Embryogenesis and Plant Regeneration System of Symplocarpus nipponicus Makino |
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| JIANG Yun-tian,CHEN Yan-qiu,ZHU Jun-yi,LI Yum-ei,QU Bai-hong.Establishment of Somatic Embryogenesis and Plant Regeneration System of Symplocarpus nipponicus Makino[J].Acta Agriculturae Nucleatae Sinica,2009,23(1):85-89. | |
| Authors: | JIANG Yun-tian CHEN Yan-qiu ZHU Jun-yi LI Yum-ei QU Bai-hong |
| Institution: | 1.Department of Biology;Tonghua Normal University;Tonghua;Jilin 134002;2.Department of Horticulture;Yanbian Agricultural College of Yanbian University;Longjing;Jilin 133400;3.Jilin Normal University;Siping;Jilin 136000 |
| Abstract: | Young leaves of Symplocarpus nipponicus Makino were used as explants in this experiment.Uniform design for the most suitable media for embryogenic callus induction and embryogenic cell complex,development of somatic embryo and plant regeneration were screened,in vitro plants had been successfully developed.The results showed that medium of LS+6-(BA 1.0mg/L)+2,4-D 2.0mg/L+IAA 0.1mg/L was suitable for embryogenic callus and embryogenic cell complex induction,the inducement percentage was 98%;the medium of dev... |
| Keywords: | Symplocarpus nipponicus Makino uniform design embryogenic cell complex somatic embryo plant regeneration |
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