YADE, a New Method for PCR Walking of Genomic DNA and cDNA in Cotton

The isolation of flanking sequences is a crucialstep in the study of gene cloning and expression.On the basis of Y-shaped adaptor(Prashar andWeissman,1996),we designed a new methodwhich could be used to amplify the flankingregion from both genomic DNA and cDNA.Thismethod was designated as Y-shaped adaptordependent extesion(YADE).The schematic ofthis method is showed in fig.1.