A comprehensive method for the determination of total potato glycoalkaloids

A method has been developed for the quantitative analysis of total glycoalkaloids (TGA) in potato tubers. The method consists of TGA extraction by suitable solvent mixtures followed by hydrolysis of the glycosides and extraction of the aglycones. The aglycones are then quantitated by nonaqueous titration. The advantages of this method over those previously described are the inclusion of glycoalkaloids that are not measured by other methods, and the simplicity, safety, and rapid nature of the procedure. This method has been applied to the TGA analysis of potato tubers subjected to a variety of storage and treatment conditions.