Disomic inheritance of anthocyanins and flavonol glycosides in the Tetraploid tuber-bearing speciesSolanum stoloniferum

Ten clones ofSolanum stoloniferum Schlechted (2n — 4x — 48) were selected from seedling populations that were produced by self-pollinating five introductions from the Commonwealth Potato Collection. These clones were used to produce S₁, F₁, F₂, and testcross populations. Clones and their progeny were scored for anthocyanins and flavonol glycosides in their corollas, stems, and leaves. Thin-layer chromatography (TLC) was used to separate and identify polyphenol compounds. No segregation for eight traits was observed within S₁ and F₁ families. Segregation was observed in F₂ and testcross populations for anthocyanins (derivatives of delphinidin and cyanidin, inhibited when the geneF is homozygous recessive); and flavonol glycosides (Q4 = quercetin 3-glucosyl-rhamnosylglucoside, controlled by the geneG1; and L1 = luteolin 7-glucoside, controlled by the geneL1) extracted from corollas. Observed ratios fitted ratios expected for disomic inheritance, differed from expected tetrasomic ratios, and provided additional evidence that the parental clones are homozygous. GenesF, G1, andL1 were genetically independent in dihybrid and trihybrid F₂ crosses.