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| 细胞冷冻保存的最佳降温速率及其影响因素 | |
| 引用本文： | 高大勇,丁力行,吕金虎,何立群. 细胞冷冻保存的最佳降温速率及其影响因素[J]. 仲恺农业技术学院学报, 2007, 20(3): 54-59 |
| 作者姓名： | 高大勇 丁力行 吕金虎 何立群 |
| 作者单位： | 1. 中国科技大学,热科学和能源工程系,安徽,合肥,230026;Department of Mechanical Engineering,University of Washington,Seattle,WA 98195,USA 2. 仲恺农业技术学院,人工环境与控制研究所,广东,广州,510225 3. 中国科技大学,热科学和能源工程系,安徽,合肥,230026 |
| 基金项目： | 中国科学院"百人计划";国家自然科学基金;仲恺农业技术学院校科研和教改项目 |
| 摘 要： | 细胞的长期保存在生物学、医学、制药等学科中具有重要意义和广泛的应用价值.细胞的低温保存对细胞产生一定的损伤作用,而其损伤机理与降温速率、细胞膜的水透过率、冷冻保护剂类型及其浓度等因素有关.文章在分析细胞冷冻保存的最佳降温速率、细胞膜水的通透性、低温保护剂及其浓度等影响因素的基础上,提出若干优化措施来防止或减轻低温损伤. |
| 关 键 词： | 细胞 冷冻保存 降温速率 冷冻损伤 |
| 文章编号： | 1006-0774（2007）03-0054-06 |
| 收稿时间： | 2007-03-10 |
| 修稿时间： | 2007-03-10 |
Optimum rate of temperature drop and its effect factors for cells'''' cryopreservation |
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| GAO Da-yong,DING Li-xing,LU Jin-hu,HE Li-qun. Optimum rate of temperature drop and its effect factors for cells'''' cryopreservation[J]. Journal of Zhongkai Agrotechnical College, 2007, 20(3): 54-59 | |
| Authors: | GAO Da-yong DING Li-xing LU Jin-hu HE Li-qun |
| Affiliation: | 1. Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, China;2. Institute of Built Environment and Control, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China;3. Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA |
| Abstract: | Long-term preservation of cells plays an important role and has a wide use in the fields of biology,medicine,and pharmacy.The process of cryopreservation brings injury to the cells,while the injury mechanism is related to many elements,such as cooling rate,permeability of cell membrane,cryoprotectant types and their concentrations.Based on the investigation of effects of the optimal cooling rate,permeability of cell membrane,cryoprotectant types and their concentrations on cell cryopreservation,some protocols were put forwards to reduce or avoid the injury due to storage at low temperature. |
| Keywords: | cell cryopreservation cooling rate cold injury |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |