Low temperature induce differential expression genes in banana fruits |
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Authors: | J.H. Caamal-Velá zquez,B.H. Chi-Manzanero,J.J. Canche-Yam,E. Castañ o,L.C. Rodrí guez-Zapata |
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Affiliation: | 1. Unidad de Biotecnología y, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Col. Chuburná de Hidalgo. C.P. 97200, Mérida, Yucatán, Mexico;2. Unidad de Bioquímica y Biología Molecular, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Col. Chuburná de Hidalgo. C.P. 97200, Mérida, Yucatán, Mexico |
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Abstract: | Differential display was used to identify gene expression of banana fruit in response to low temperature stress. Banana fruits were kept at 10 °C for 8 h and 60 differential expressed fragments in pulp and peel were obtained. These fragments had an average size of 200 pb or greater and Dot blotting hybridization as well as Northern blot corroborated specific expression of these differential expressed fragments. Among the several differentially expressed genes, we found genes involved in response to pathogen attack, wounding and a ripening-associated gene. We consider these genes to belong to a general pathway which is activated upon general stress signaling. |
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Keywords: | Bananas cDNA Low temperature injury Differential display Musa acuminata |
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