Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media |
| |
Authors: | Liljana R. Koleva-Gudeva Mirko Spasenoski Fidanka Trajkova |
| |
Affiliation: | 1. Institute of Southern Crops, Department of Biotechnology, Goce Delcev b.b., 2400 Strumica, The Republic of Macedonia;2. Faculty of Natural Science and Mathematics, Institute of Biology, Arhimedova 5, P.O. Box 162, 1000 Skopje, The Republic of Macedonia |
| |
Abstract: | The frequency of obtained androgenic plants depends highly on the genotype; therefore the low rate of haploid recovery limits the utility of anther culture in pepper breeding. The need for incubation treatment and adequate nutrition media supplemented with plant growth regulators, especially auxins, are suggested as important factors to obtain somatic haploid embryos in pepper anther culture. The effect of three incubation treatments of the androgenic potential in pepper anther culture on MS, N, LS, NN and CP medium are summarised, and the results demonstrate that:- •
- by incubating treatment in cold conditions (at 7 °C) in darkness for 7 days, and then transferring the explants to light conditions (12-h photoperiod at 25 °C) for 4 weeks, on LS and NN mediums, anthers produced callus;
|
| |
Keywords: | Androgenesis Thermal shock Embryo formation In vitro Capsicum annuum L |
本文献已被 ScienceDirect 等数据库收录! |
|