| 农杆菌介导的甘蔗遗传转化研究 |
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| 引用本文： | 陈如凯,林俊扬,林俊芳,郑学勤.农杆菌介导的甘蔗遗传转化研究[J].亚热带农业研究,1996(3). |
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| 作者姓名： | 陈如凯 林俊扬 林俊芳 郑学勤 |
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| 作者单位： | 福建农业大学甘蔗综合研究所，福建省莆田市农业科学研究所，中国热带农业科学院热带作物生物技术国家重点实验室 |
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| 摘 要： | 选用在组织培养过程中能够产生分泌较多酚类物质的甘蔗基因型福农８６／１７为材料．以幼叶切段为外植体，采用叶盘法与土壤根癌农杆菌菌珠ＬＢＡ４４０４（ｐＡＬ４４０４：：ＰＴＤ１）菌液并培养。外植体设预培养１天、３天、５天、７天和１０天五种时间，农杆菌设稀释１０倍和２０倍两种浓度，共１０种处理组合，共培养４８小时。结果预培养５天，农杆菌稀释１０倍和２０倍两种处理组合的外植体．在含卡那霉素３００ｍｇ／Ｌ的选择诱导和选择继代培养基中诱导并形成抗性愈伤组织。
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| 关 键 词： | 甘蔗，农杆菌，遗传转化 |
Experiment on the Sugarcane Genetic Transformation Mediated by Agrobacterium tumefaciens |
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| Abstract: | Abstract The leaf meristem cuts of sugarcane genotype Funong 86/17 was selected as explants and coincubated with Agrobacterium tumefaciens strain LBA 4404 (pAL 4404:pTD1) by leaf disc for 48 hours.Ten treatments were designed at 5 explant precultures of 1,3, 5, 7,10 days and 2 Agrobacterium tumefaciens concentrations of 10, 20 folds.The result was that the calli resistant to kanamycin were induced and produced from the explants of 2kinds of treatments on the selection inductive and selection subculture media containing 300mg/L of kanamycin,one treatment was 5 days of preculture and 10 folds of Agrobacterium tumefaciens concentration,another was 5 days of preculture and 20 folds of A.tumefaciens concentration. |
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