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应用15N稀释法测定土壤氮素初级转化速率的一些关键技术
引用本文:王 敬,张金波,蔡祖聪. 应用15N稀释法测定土壤氮素初级转化速率的一些关键技术[J]. 土壤, 2016, 48(3): 429-433
作者姓名:王 敬  张金波  蔡祖聪
作者单位:南京师范大学地理科学学院,南京师范大学地理科学学院,南京师范大学地理科学学院
基金项目:国家自然科学基金重点项目(41330744),江苏省普通高校学术学位研究生科研创新计划项目(KYZZ15_0218)和江苏高校优势学科建设工程(PAPD,164320H116)资助。
摘    要:本文综合评述了应用~(15)N库稀释法测定土壤氮素初级转化速率的一些关键技术,即~(15)N标记土壤氮库的方法、~(15)N的加入量、丰度和标记物种类的选择,以及初始取样时间的确定。只有合理地运用这些关键技术,才能更准确地测定土壤氮素初级转化速率,进而更真实地表征土壤氮素的实际周转状况。

关 键 词:15N稀释法;15N均匀分布;15N加入量;15N丰度
收稿时间:2015-04-01
修稿时间:2015-04-29

Several Key Techniques in Calculating Gross N Transformation Rates by 15N Dilution Methods
WANG Jing,ZHANG Jinbo and CAI Zucong. Several Key Techniques in Calculating Gross N Transformation Rates by 15N Dilution Methods[J]. Soils, 2016, 48(3): 429-433
Authors:WANG Jing  ZHANG Jinbo  CAI Zucong
Affiliation:School of Geography Sciences,Nanjing Normal University,School of Geography Sciences,Nanjing Normal University and School of Geography Sciences,Nanjing Normal University
Abstract:Several key techniques in calculating gross N transformation rates by 15N dilution methods were reviewed in this paper. Such techniques include methods of applying 15N to soil, amount, enrichment and types of 15N addition, and initial sampling time. Only when these techniques were applied reasonably under particular conditions, 15N dilution methods could be successfully to quantify gross N transformation rates and to understand the fundamental processes that regulate individual microbial N pathways.
Keywords:15N dilution methods   Uniform distribution of 15N   Amount of 15N addition   Enrichment of 15N
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