Immunochemical studies on a Brucella ovis specific protein antigen |
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| Authors: | C E Suarez G A Pacheco A M Vigliocco |
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| Affiliation: | Comision Nacional de Energia Atomica, Buenos Aires, Argentina. |
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| Abstract: | A Brucella ovis surface protein antigen (P-II), obtained by gel filtration with Sepharose 4B of a hot saline extract was characterized. The analysis of P-II over gradient sodium dodecylsulfate electrophoresis yielded an 18.5 and a 20 kDa band. In a radioimmunoprecipitation assay using P-II labeled with 125I, the antigen reacted specifically only with sera from rams experimentally infected with a naturally occurring rough strain of B. ovis and did not react with sera from rams experimentally infected with other smooth Brucella strains (B. abortus and B. melitensis). |
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