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Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop |
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| Authors: | M D Pescovitz B K Book B Aasted J Dominguez R Bullido I Trebichavsky B Novikov I Valpotic M Tomaskovic J Nielsen S Arn D H Sachs J K Lunney P C Boyd J Walker R Lee A Saalmüller |
| Institution: | a Indiana University, Dept. of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USA b Royal Veterinary and Agricultural University, Frederiksberg C, Denmark c Centro de Investigaciones en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain d Institute Microbiology, Prague, Czech Republic e All-Russian Research Institute of Veterinary Virology and Microbiology, Vlasimizskaya obl., Russian Federation f Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, Croatia g Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark h Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USA i Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, MD, USA j Center for Animal Biotechnology, University of Melbourne, Victoria, Australia k Federal Research Center for Virus Diseases of Animals, Tübingen, Germany |
| Abstract: | Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAbs fell within cluster T13 including the CD5a standard b53b7 (No. 174). The two new mAbs 1H6/8 (No. 058) and BB6-9G12 (No. 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAbs b53b7 to peripheral lymphocytes. These mAbs, therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAbs to eight, all of which appear to recognize the same epitope. |
| Keywords: | Monoclonal antibodies CD5 T-cell Porcine |
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