12个楸树无性系叶片多酚含量与抗氧化活性

自由基具有强氧化性,机体衰老及多种疾病如肿瘤、炎症、神经退行性疾病、动脉粥样硬化等的诱发促进机制都与自由基有关[1]。自由基的减少,不仅要靠自身的清除系统,还需要外部抗氧化剂的协助。近年来,关于合成抗氧化剂的副作用受到人们更多的关注,因此,寻找安全、高效的植物源类抗氧化剂,已成为食品领域研究的热点。楸树(Catalpa bungei C.A.Mey.)为紫葳科(Bignoniaceae)梓属植物,落叶乔木,《本草纲目》记载楸树树皮、叶具有药

Study on Polyphenol Content and Antioxidant Activity from Twelve Clones of Catalpa bungei Leaves

In this study, the antioxidant activity of 12 Catalpa bungei clones extracted from He'nan and Gansu was evaluated in order to find the most effective ones with higher antioxidant activity as well as total phenol content. The results showed that CA6 from Gansu Province was the most effective one in reducing ability (41.61%), DPPH radical scavenging activity (the reciprocal of IC₅₀), hydroxyl radical scavenging activity (the reciprocal of IC₅₀) and the highest total phenol content (31.73 mg·g^-1) among the 12 clones. The reducing ability and total phenol content determined in 6 clones in Gansu were significantly higher than that in He'nan Province. In addition to CA3, the hydroxyl radical scavenging activity and DPPH radical scavenging activity of the other 5 clones in Gansu were significantly higher than in that He'nan Province. Moreover, the antioxidant activity of ethyl acetate fraction in CA6 leaves was the highest, followed by n-butanol, petroleum ether, and the water fractions was the lowest.