猪体内筛选RHDV VP60 T细胞表位的优势区

应用戊二醛醛化的人＂O＂型红细胞纯化兔出血症病毒（RHDV）,对非免猪进行3次免疫后,分离外周血淋巴细胞经不同段重组杆状病毒（共分5段表达）、纯化的病毒、Con A刺激后进行IFN-γ、IL-2、IL-4、WST检测。结果显示,纯化病毒的血凝效价为28,与未纯化的RHDV毒价相差1个滴度;WST检测结果为免疫组经3段重组杆状病毒刺激的值最高;IFN-γ和IL-2检测结果也是3段重组杆状病毒刺激的值最高;IL-4检测结果为3段重组杆状病毒刺激的值最低。由此表明病毒经纯化后效果好,3段重组杆状病毒区域为T细胞表位优势区,这为RHDV T细胞表位的进一步研究奠定基础。

Screening specific T lymphocyte epitopes superiority region of VP60 protein of rabbit hemorrhagic disease virus in pig

The pigs were inoculated three times with the rabbit hemorrhagic disease virus(RHDV) purified by preparation of hydroformylated human O-type red blood cell to detect IFN-γ,IL-2,IL-4,WST after sterility isolation blood lymphocyte stimulated by various rebaculoviruses,purified RHDV and Con A.The hemagglutination valency of purified virus was 28,in correspondence with the unpurified virus.The statistically analysis was performed using SPSS software packages.The NO.3 rebaculoviruses was the highest quantities of WST producing than the others(P<0.01).In addition,the NO.3 rebaculoviruses was the highest quantities of IFN-γ and IL-2 producing than the others,but was the lowest quantities of IL-4 producing than the others.It is demonstrated that efficiency of purified RHDV was high and the NO.3 rebaculoviruses contain T-cell epitopes.