黄鳝精子活力检测和精子入卵早期过程观察

采用Olympus3×51相差系统显微镜和SQIAS—1000彩色精液质量图文分析系统检测黄鳝精子活力。结果表明,在NaCl溶液浓度为0～0.3%时,黄鳝精子激活比例随溶液浓度升高而极显著增加(P<0.01);当NaCl浓度达到0.7%时,精子激活比例、直线运动速度和鞭毛摆动频率均显著(P<0.05)或极显著(P<0.01)降低。扫描电镜观察显示:黄鳝成熟卵卵壳膜上的精孔区呈漏斗状凹陷,其底部中央可见一精孔管外孔,口径约4.22±0.66μm;黄鳝精子入卵速度缓慢,受精过程较长,从精子附着于卵球表面到精孔管完全堵塞,约30s～5min。

The sperm motility and initial stages of penetration of sperm into eggs in Monopterus albus

The sperm motilities were determined by using phase contrast microscope and analytical system of computer. The result s show the sperm activity rate signif icant ly increases( P < 0. 01) w ith the concentration of NaCl when that of NaCl ranges from 0 to 0. 3% in Monopterus albus, but the sperm activity rate, linear velocity of the spermatozoal mot ion and the frequency of flagellar beat of spermatozoa signif icantly reduce ( P < 0. 05) when the concentration of NaCl exceeds 0. 7% . The micropylar region of the mature egg is like a funnel cave in M. albus under scanning electron microscopy. A micropylar canal is located at the central section of the bottom of the cave, about 4. 22 .. 0. 66 ..m in diameter. The penetration of sperm into the eggs is slow and the process of the fertilization is long. The sperm originally arrives at the surface of the chorion of M. albus 30s after insemination. The micropylar canal is blocked by the fertilized plug within 5 minutes after insemination.