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| 柔嫩艾美耳球虫端粒DNA重复序列的South-ern印迹杂交分析 | |
| 引用本文： | 杨桂连,李建华,张西臣,赵权,宫鹏涛,蔡亚南. 柔嫩艾美耳球虫端粒DNA重复序列的South-ern印迹杂交分析[J]. 吉林农业大学学报, 2010, 32(2): 205-208 |
| 作者姓名： | 杨桂连 李建华 张西臣 赵权 宫鹏涛 蔡亚南 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,长春130062;吉林农业大学动物科学技术学院,长春130118 2. 吉林大学畜牧兽医学院,长春,130062 3. 吉林农业大学动物科学技术学院,长春,130118 |
| 基金项目： | 国家自然科学基金项目(30671580,30170696) |
| 摘 要： | 为进一步确定柔嫩艾美耳球虫(E.tenella)端粒DNA的重复序列,为下一步端粒酶活性检测奠定基础,根据已克隆发表的E.tenella端粒DNA重复序列信息,设计了由4个端粒重复序列串联的寡聚核苷酸探针(TTTAGGG)4,并以生物素地高辛标记。将该探针与经BAL31-EcoRⅠ酶切后的E.tenella基因组DNA进行Southern印迹杂交分析。结果显示:E.tenella基因组DNA与探针杂交获得了清晰的杂交条带,随BAL31酶切时间的延长,杂交信号逐渐减弱,进一步证明了E.tenella端粒DNA重复序列为5-′TTTAGGG-3′,且此重复序列在E.tenella染色体的末端。 |
| 关 键 词： | 柔嫩艾美耳球虫 端粒DNA Southern印迹杂交 |
| 收稿时间： | 2009-04-09 |
| 修稿时间： | 2009-07-14 |
Southern Blotting Analysis of Telomeric Repetitive DNA Sequences in Eimeria tenella |
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| YANG Gui-lian,,LI Jian-hua,ZHANG Xi-chen,ZHAO Quan,GONG Peng-tao,CAI Ya-nan.College of Animal Science , Veterinary Medicine,Jilin University,Changchun ,China,.College of Animal Science , Technology,Jilin Agricultural University,Changchun. Southern Blotting Analysis of Telomeric Repetitive DNA Sequences in Eimeria tenella[J]. Journal of Jilin Agricultural University, 2010, 32(2): 205-208 | |
| Authors: | YANG Gui-lian LI Jian-hua ZHANG Xi-chen ZHAO Quan GONG Peng-tao CAI Ya-nan.College of Animal Science Veterinary Medicine Jilin University Changchun China .College of Animal Science Technology Jilin Agricultural University Changchun |
| Affiliation: | YANG Gui-lian1,2,LI Jian-hua1,ZHANG Xi-chen1,ZHAO Quan2,GONG Peng-tao1,CAI Ya-nan11.College of Animal Science , Veterinary Medicine,Jilin University,Changchun 130062,China,2.College of Animal Science , Technology,Jilin Agricultural University,Changchun 130118 |
| Abstract: | In order to identify E.tenella telomeric repetitive DNA sequences and detect the activity of telomerase,an oligonucleotide probe composed of (TTTAGGG)4 telomeres was designed according to the E.tenella telomeric repetitive DNA sequence (5′-TTTAGGG-3′) which had been previously cloned in our laboratory and labeled by digoxin.The probe was hybridized with the products of E.tenella genomic DNA digested with BAL31 and EcoRⅠand then analyzed by Southern blotting.The results indicated that the hybridization of the probe and the E.tenella genomic DNA produced clear bands.With the delay of BAL31 digestion,hybridization signal decreased gradually.It further proved that E.tenella telomeric repetitive DNA sequences were 5′-TTTAGGG-3′and located in the terminal region of E.tenella chromosome. |
| Keywords: | Eimeria tenella telomeric DNA Southern blotting |
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