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Analysis on Membrane Stability of Crown in Winter Wheat in Frigid Region in China
Authors:Wang  Xiao-nan Xie  Dong-wei Fu  Lian-shuang Sun  Ying-lu Li  Zhuo-fu Luan  Fei-shi
Institution:College of Agricalture,Northeast Agricultural University
Abstract:Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde (MDA) content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase (SOD), peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione (GSH) content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties.
Keywords:winter wheat  cold resistance  cell viability  protective enzyme  plasma membrane peroxidation  water
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