利用JIVET技术快速繁育萨福克羊的研究

JIVET技术是一项利用生殖激素刺激幼畜卵巢,从而使其快速、高效生产卵母细胞的技术。对4只1月龄萨福克幼羔注射FSH和PMSG激素,48 h后采用手术法抽取幼羔卵母细胞,并在体外成熟;将成熟的卵母细胞与精子进行体外受精,将正常分裂的2~8细胞胚胎移植到6只同期发情的受体小尾寒羊子宫内。结果显示,采用激素法处理萨福克幼羔后共采集到可用卵母细胞109枚,体外成熟培养后得到成熟卵母细胞79枚,成熟率为72.48%;体外受精后得到正常卵裂胚胎34枚,卵裂率为43.04%;胚胎移植后有3只受体母羊怀孕,受胎率为50%,并分娩得到3只萨福克幼羔。综上提示,利用JIVET技术可以缩短萨福克羊的繁育周期,从而加快其繁育速度。

Study on Rapid Propagation of Suffolk Sheep by Using JIVET Technology

JIVET is a technique using reproductive hormone to stimulate ovarian of young stock to rapidly and efficiently produce oocytes. In this study, in order to evaluate the application performance of JIVET in rapid propagation of Suffolk Sheep, 4 one-month-old lambs of Suffolk Sheep were selected and intramuscularly administrated with FSH and PMSG. Forty-eight hours after administration of reproductive hormone, the oocytes were collected by surgery and were subsequently matured in vitro. The matured oocytes were fertilized with prepared frozen semen in vitro. When the embryos grew to 2 to 8 cells each, they were transferred into the uterus of 6 receptor female sheep. The results revealed that a total of 109 available oocytes were obtain after the treatment of reproductive hormone and 79 of them were successfully matured in vitro, and the maturation rate was 72.48%. A total of 34 cleaved embryos were obtained after fertilization in vitro and the cleavage rate was 43.04%. Three receptor female sheep was pregnant after receiving embryo transfer and the pregnancy rate was 50%. Finally, 3 lambs of Suffolk Sheep were obtained. These results suggest the fact that the application of JIVET can shorten the breeding cycle of Suffolk Sheep and thus speed up the breeding of Suffolk Sheep.