牛蛙不同体部皮肤的组织学研究

通过制作石蜡切片,并利用苏木精—伊红染色法进行染色,对牛蛙不同部位的皮肤进行组织学观察;利用图像处理软件Image J 4.21测量皮肤厚度,运用SPSS 17.0软件对不同部位的皮肤厚度进行单因素方差分析。镜下观察组织切片可见,牛蛙的皮肤分为表皮和真皮,表皮由外向内可分为3层：角质层、颗粒层和生发层,真皮由疏松层和致密层组成。疏松层主要由网状结缔组织构成,也包括一些色素、颗粒腺和黏液腺。色素主要分布于表皮下的结缔组织中,颗粒腺和黏液腺的间隙的结缔组织中也有少量色素。鼓膜皮肤厚度显著小于其他部位皮肤厚度（P〈0.05）,前肢、后肢、泄殖孔的皮肤厚度差异不显著（P〉0.05）。不同部位皮肤分布的色素细胞、颗粒腺、黏液腺数量也不同,内侧的黏液腺多于外部,而颗粒腺在表皮分布多,在深层分布少。

Histological Study on Skin in Different Body Parts of Rana catesbeiana

Abstract ：The skin in different body parts of Rana catesbeiana were collected to prepared paraffin sections by using HE staining method and were histologically observed. The thickness of skin in different body parts was determined by using image processing software of hnage J 4.21 and was statistically analyzed by One-Way ANOVA analysis of variance using SPSS 17.0 statistical software. The skin of Rana cazesbeiana consisted of epidermis and dermis. The epidermis could be divided into 3 layers from outside to inside, including cuticle, granular layer and germinal layer. The dermis was composed of loose layer and dense layer. The loose layer was primarily composed of reticular connective tissue and also included some pigments, granular glands and mucous glands. The pigments were mainly distributed in subcuticular connective tissue, and a small amount of pigments were distributed in the connective tissue in gaps between granular glands and mucous glands. The value of thickness of tympanic skin was significantly lower than that of skin in the other parts of Rana catesbeiana （P〈0.05）. No significant differences in thickness of skin in forelimb, hind limb and cloacal pore were observed （P〉0.05）. The distributing density of pigment cells, granular glands and mucous glands varied in skin in different body parts of Rana catesbeiana. The number of mucous glands distributing inside was more than that outside, and the granular glands were mainly distributed in epidermis but barely in deep layer.