不同浓度玻璃化冷冻保护液及冷冻载体对驴卵母细胞成熟率的影响

为了比较不同冷冻液及冷冻载体对驴卵母细胞成熟率的影响,试验设计4个浓度梯度（Ⅰ、Ⅱ、Ⅲ、Ⅳ）冷冻液和3种冷冻载体分别对生发泡（germinal vesicle,GV）期驴的卵母细胞进行玻璃化冷冻,对解冻后的卵母细胞进行体外成熟,通过比较卵母细胞的成熟率反映其冷冻效果。结果表明,冷冻液Ⅱ的卵母细胞成熟率（28.13%）显著（P〈0.05）高于其他3组,但显著（P〈0.05）低于正常组（51.77%）,在不同冷冻载体对驴卵母细胞成熟率的影响试验中,使用开放式拉长塑料细管（OPS）的卵母细胞成熟率（28.13%）显著（P〈0.05）高于普通玻璃细管组（11.67%）,但与GMP管组（23.53%）差异不显著（P〉0.05）。

Effect of Different Vitrificated Cryopreservation Solutions and Loading Devices on in vitro Maturation Rate of Donkey Oocytes

The aim of the present study was to evaluate the effect of different vitrificated cryopreservation solutions and loading devices on in vitro maturation rate of donkey oocytes. Four concentration gradient of solutions（ Ⅰ, Ⅱ, Ⅲ, Ⅳ） and 3 sets of loading devices were included in this study. The GV-stage oocytes of donkey were cryopreserved by using different solutions and loading devices and were subsequently matured in vitro after thawing. The maturation rate of donkey oocytes was served as an index to compare their performance in cryopreservation. The results demonstrated that the maturation rate of oocytes cryopreserved by solution Ⅱ（28.13%） was significantly higher than that of oocytes cryopreserved by the other 3 solutions（P0.05）, but was significantly lower than that of oocytes in control（51.77%,P0.05）. The maturation rate of oocytes cryopreserved by OPS（28.13%） was significantly higher than that of oocytes cryopreserved by ordinary glass straws（11.67%,P0.05）, but was not significant different compared with that of oocytes cryopreserved by GMP（23.53%,P0.05）.