含N-乙酰半胱氨酸稀释液对鸡精液低温保存效果的影响

采集安卡红种公鸡鲜精,分别用含不同浓度N-乙酰半胱氨酸的A、B 2种稀释液稀释后低温保存,之后测定保存时间、生存指数和精子畸形率,比较不同浓度的N-乙酰半胱氨酸对鸡精液在低温保存下的效果。结果表明：A液中,0.013 0 mol/L添加量和0.002 6 mol/L添加量相比保存时间和生存指数均无显著差异（P〉0.05）,且均极显著（P〈0.01）高于A液无添加组;B液中,0.013 0 mol/L添加量在保存时间和生存指数方面均极显著（P〈0.01）优于0.002 6 mol/L添加量和无添加组,0.002 6 mol/L添加量与B液无添加组相比,保存时间和生存指数差异均不显著（P〉0.05）。而2种稀释液中是否添加N-乙酰半胱氨酸及添加量的多少对精子畸形率影响均不大（P〉0.05）。

Effect of Addition of N-acetyl-cysteine in the Extender on Cryo-preservation Quality of Chicken Semen

This study was carried out to evaluate the effect of supplementing different concentrations of N-acetyl-cysteine in the extender on cryo-preservation quality of chicken semen. The fresh semen samples from Anka breeder chickens were collected and diluted with two separate extenders （A and B） each adding different concentrations （0.013 0 and 0.002 6 mol/L） of N-acetyl- cysteine. The diluted semen samples were subsequently cryo-preserved and the preservation time, survival index and deformity rate were determined. The results revealed that in the A extender, there were no significant differences in preservation time and survival index between the 0.013 0 mol/L of N-aeetyl-cysteine treatment samples and 0.002 6 mol/L of N-acetyl-cysteine treatment samples （P〉0.05）, but preservation time and survival index of the two N-acetyl-cysteine treatment samples were significantly higher than those of control （P〈0.01）; in the B extender, the preservation time and survival index of the 0.013 0 mol/L of N-aeetyl-eysteine treatment samples were significantly higher than those of 0.002 6 mol/L of N-acetyl-cysteine treatment samples and control （P〈0.01）, but no significant differences in preservation time and survival index between the 0.002 6 mol/L of N-acetyl-cysteine treatment samples and control were observed （P〉0.05）. The addition and adding levels of N-acetyl-cysteine in the extenders had no obvious effect on the sperm deformity rate of Anka breeder chickens （P〉0.05）.