| 绵羊肺炎支原体的培养和PCR鉴定 |
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| 引用本文： | 绵羊肺炎支原体的培养和PCR鉴定.绵羊肺炎支原体的培养和PCR鉴定[J].畜牧与饲料科学,2016,37(11):97-97. |
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| 作者姓名： | 绵羊肺炎支原体的培养和PCR鉴定 |
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| 作者单位： | 安徽省农业科学院畜牧兽医研究所,安徽合肥230031 |
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| 基金项目： | 安徽省农业科学院学科建设项目（16A0413）; 国家自然科学基金项目（31402048） |
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| 摘 要： | 安徽省3个山羊场有羊只发生肺炎症状,用支原体培养基对病料进行病原分离,经传代和纯化后分离到3株支原体,随后对分离到的支原体进行了形态学观察、PCR鉴定和序列比对,结果显示分离到的支原体均为绵羊肺炎支原体。
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Culture and PCR Identification of Mycoplasma ovipneumoniae |
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| Abstract: | Pneumonia symptoms of goats appeared in three goat farms in Anhui Province. The mycoplasma isolate was cultivated and purified from lung tissue sample of goats using mycoplasma medium. The mycoplasma isolate was characterized by morphologic observation, PCR identification and sequence alignment. The results indicate that the mycoplasma isolate belongs to Mycoplasma ovipneumoniae. |
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