精氨酸对妊娠后期鄂尔多斯细毛羊胎儿生长发育的影响研究

旨在通过对鄂尔多斯细毛羊母羊颈静脉灌注L-精氨酸,研究其对鄂尔多斯细毛羊母羊妊娠后期（90-140d）胎儿生长与发育的影响。试验选择22只母羊,在90 d开始灌注115μmol L-Arg-HCl/kg体重（Arg1组）、155μmol L-Arg-HCl/kg体重（Arg2组）和生理盐水（对照组）,3次/d。妊娠第115天和第140天,进行实验母羊孕体检查,并记录胎儿体重、器官重量和体尺长度,收集母体及胎儿血浆测定血浆氨基酸浓度。结果表明,母羊妊娠第140天,Arg2组较Arg1组和对照组显著增加了胎儿心脏和肝脏重量（P〈0.05）。母羊妊娠第115天和第140天,灌注L-精氨酸显著增加母体血浆精氨酸和鸟氨酸浓度（P≤0.01）,增加胎儿血浆生糖氨基酸（苏氨酸、甲硫氨酸、苯丙氨酸和赖氨酸）浓度（P≤0.05）。

Effects of Arginine on Growth and Development of Ordos Fine-wool Sheep Fetuses during Late Gestation

The objective of this study was to evaluate the effects of L-arginine infusion through jugular vein on growth and development of Ordos Fine-wool Sheep fetuses during late gestation. Twenty-two ewes received an i.v.bolus of either 115μmol/（kg·BW） of Arg-HCl（Arg1 group） or 155 μmol/（kg·BW） of Arg-HCl（Arg2 group） or saline solution（control group） 3times per day from 90 to 140 days post-pregnancy. On the days 115 and 140 of pregnancy, the ewes were physically examined;The body weight, organ weight and body length of their fetuses were recorded; The concentrations of amino acids in plasma of ewes and fetuses were determined. The results revealed that on the day 140 of pregnancy, the heart weight and liver weight of fetuses in Arg2 group were significantly increased compared with those of fetuses in Arg1 group and control group（P〈0.05）. On the days115 and 140 of pregnancy, the plasma concentrations of arginine and ornithine of ewes received systematic L-arginine infusion were significantly increased（P ≤0.01）, and the plasma concentrations of glucogenic amino acid of their fetuses, including threonine, methionine, phenylalanine and lysine, were also significantly increased（P≤0.05）.