|
|||||
|
|
| 2株猪圆环病毒2型ORF2的克隆及序列分析 | |
| 引用本文： | 辜吉秀,刘艳红,尹双辉,宋冰,刘湘涛.2株猪圆环病毒2型ORF2的克隆及序列分析[J].动物医学进展,2008,29(5):4-6. |
| 作者姓名： | 辜吉秀 刘艳红 尹双辉 宋冰 刘湘涛 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州730070;中国农业科学院兰州兽医研究所,甘肃兰州,730046 2. 中国农业科学院兰州兽医研究所,甘肃兰州,730046 3. 甘肃农业大学动物医学院,甘肃兰州,730070 |
| 基金项目： | 国家科技支撑计划 , 甘肃省农业生物技术研究与应用开发项目 |
| 摘 要： | 根据GenBank中猪圆环病毒2型(PCV-2)的核酸序列,设计一对引物,采用PCR方法从疑似断奶仔猪多系统衰竭综合征(PMWS)的死亡仔猪组织病料中扩增出ORF2基因,将其克隆到pMD18-T载体上,筛选获得重组质粒pMD18T-ORF2。序列分析表明,克隆的ORF2基因与其他PCV-2ORF2的分离株的同源性在91.5%～99.4%,与杭州分离株的同源性达到99.4%,表明我国各地区之间PCV-2的分离株存在较大的差异。 |
| 关 键 词： | 猪圆环病毒2型 ORF2基因 克隆 序列分析 |
| 文章编号： | 1007-5038(2008)05-0004-03 |
| 修稿时间： | 2008年2月2日 |
Cloning and Sequence Analysis of ORF2 Gene of Two PCV-2 Strains |
|
| GU ji-xiu,LIU Yan-hong,YIN Shuang-hui,SONG Bing,LIU Xiang-tao.Cloning and Sequence Analysis of ORF2 Gene of Two PCV-2 Strains[J].Progress In Veterinary Medicine,2008,29(5):4-6. | |
| Authors: | GU ji-xiu LIU Yan-hong YIN Shuang-hui SONG Bing LIU Xiang-tao |
| Abstract: | Using a pair of specific primers designed according to published ORF2 gene sequence of PCV-2,the ORF2 gene was amplified from the suspected PMWS samples by PCR.The amplified fragments were cloned into the pMD18-T vectors and the positive recombinant plasmids were designated PMD18-T-ORF2.The results indicated that it share 91.5%to 99.4% homology at their nucleotide level.There has only 91.5% nucleotide identity with Henna isolates and shared most high sequence identity of 99.4% with Zhejiang isolate,99.4%.The data indicated that there have genetic differences from different geographical regions. |
| Keywords: | PCV-2 ORF2 gene cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |