| 褐点粉灯蛾核型多角体病毒的研究一核酸的纯化和特性 |
| |
| 引用本文： | 孙松柏,陈明树,李映萍,林均武. 褐点粉灯蛾核型多角体病毒的研究一核酸的纯化和特性[J]. 西南林业大学学报,1985,5(1): 55-63.doi:10.11929/j.issn.2095-1914.1985.01.008 |
| |
| 作者姓名： | 孙松柏 陈明树 李映萍 林均武 |
| |
| 作者单位： | 1.中国科学院武汉病毒研究所;2.西南林学院 |
| |
| 摘 要： | 我国对灯蛾科昆虫病毒研究甚少,文献记载仅有二种;美国白蛾(Hyrhantria cunea)核型多角体毒病,红缘灯蛾（Amsacta lactinca)颗粒体病毒研究仅限于病毒形态观察及生物学特性等方面,而对于病毒核酸的研究均未见报道。
|
Study on the nuclear polyhedrosis virus of Alphaea phasma Leech(ApNPV)-purification and characterization of ApNPY DNA |
| |
| Sun Songbai,Chen Mingshu,Li Yingping and Lin Junwu. Study on the nuclear polyhedrosis virus of Alphaea phasma Leech(ApNPV)-purification and characterization of ApNPY DNA[J]. Journal of Southwest Forestry University,1985,5(1): 55-63.doi:10.11929/j.issn.2095-1914.1985.01.008 |
| |
| Authors: | Sun Songbai Chen Mingshu Li Yingping Lin Junwu |
| |
| Affiliation: | 1.Wuhan Institute of Viroloy,Academia Sinica;2.South-western Forestry College |
| |
| Abstract: | In this paper,report the purification and some characterization of ApNPV(virus particles and DNA.) After alkali-treatment polyhedra with 0.05M Na2CO3-0.05M NaCl,PH 10.8,(electron micrograph revealed) it was observed under electron microscope that the size of the virions/averages 55×333nm,and the size of the polyhedra averages 1.66μ.ApNPV DNA was isolated from ApNPV(virus particles multiply in the polyhedral matrix) after treated with TritonX-100,NaCl,DNase,RNase and pronase deproteinized by SDS-phenol method.Some of their physical properties were determined.The ApNPV DNA is a circular double stranded DNA molecule.Electron microscopic observation to this DNA preparation makes clear that the mean outer girth is about 16.8-32μ. The restriction endonuclease,EcoR Ⅰ,Bgl Ⅰ cleaved the ApNPV DNA 11,13 fragments respectively.By using molecule weight standard determining methods,the determining result is that the mol.wt.of ApNPV DNA is about 60×106 dalton. |
| |
| Keywords: | |
|
| 点击此处可从《西南林业大学学报》浏览原始摘要信息 |
|
点击此处可从《西南林业大学学报》下载全文 |
|
