| PRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用 |
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| 引用本文： | 徐引弟,王治方,朱文豪,张青娴,焦海燕,郭成留. PRRSV变异株与普通株二重RT-PCR鉴别方法的建立与应用[J]. 河南农业科学, 2009, 0(9) |
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| 作者姓名： | 徐引弟 王治方 朱文豪 张青娴 焦海燕 郭成留 |
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| 作者单位： | 河南省农业科学院,畜牧兽医研究所,河南,郑州,450002 |
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| 基金项目： | 河南省科技攻关计划项目，河南省农科院高技术产品孵化项目 |
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| 摘 要： | 参考GenBank发表的猪繁殖与呼吸综合征病毒(PRRSV)的nsp2基因序列,在nsp2大缺失区下游的保守区设计了1条下游引物,在小缺失区设计1条上游引物,用于变异株的扩增。在大缺失区内部设计1条上游引物,与变异株共用下游引物,用于普通株的扩增,建立了PRRSV变异株与普通株二重RT-PCR鉴别方法。建立的RT-PCR方法用于临床病料的检测,部分阳性结果测序,变异株序列与报道的序列同源性在97%以上。结果表明,该方法特异、快速、简便,可以用于PRRSV变异株和普通株的鉴别。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 二重RT-PCR 鉴别方法 |
Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common Strains |
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| XU Yin-di,WANG Zhi-fang,ZHU Wen-hao,ZHANG Qing-xian,JIAO Hai-yan,GUO Cheng-liu. Development and Application of A Duplex RT-PCR Method to Differentiate the PRRSV Variant and Common Strains[J]. Journal of Henan Agricultural Sciences, 2009, 0(9) |
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| Authors: | XU Yin-di WANG Zhi-fang ZHU Wen-hao ZHANG Qing-xian JIAO Hai-yan GUO Cheng-liu |
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| Affiliation: | Institute of Animal Husbandry and Veterinary Research;Henan Acadmy of Agricultural Sciences;Zhengzhou 450002;China |
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| Abstract: | According to the nsp2 gene sequence of PRRSVs in GenBank,a downstream primer was designed in the conserved region of the downstream of large deletion of nsp2 and a upstream primer in the short deletion to amplify the variant strain.Common strain was amplified using a upstream primer within the large deletion and the same downstream primer to variant strain.A duplex RT-PCR distinguish method to differentiate the PRRSV variant and common strains was development.This method was applied to test clinic samples.P... |
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| Keywords: | PRRSV Duplex RT-PCR Differential method |
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