| Dot-ELISA检测兔疥螨抗体方法的建立和应用 |
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| 引用本文： | 郝桂英,杨光友,古小彬. Dot-ELISA检测兔疥螨抗体方法的建立和应用[J]. 动物医学进展, 2009, 30(4) |
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| 作者姓名： | 郝桂英 杨光友 古小彬 |
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| 作者单位： | 1. 四川农业大学动物医学院,四川雅安,625014;西昌学院动物科学系,四川西昌,615013
2. 四川农业大学动物医学院,四川雅安,625014 |
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| 摘 要： | 将从病兔痂皮内收集的疥螨经研磨、冻融、离心后,制成可溶性抗原,作为诊断抗原,建立Dot-ELISA方法检测兔疥螨血清抗体.研究确定了该方法的最佳工作条件.制备的诊断膜片特异性强,不与兔瘟病毒、兔大肠埃希菌、兔附红细胞体等阳性血清反应.膜片具有良好的灵敏性,高免血清作1∶210稀释亦能检出;重复性试验表明该法重复性良好.诊断膜片在4 ℃保存5个月其检测活性不变.结果表明,建立的Dot-ELISA可用于免疥螨抗体的检测.
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| 关 键 词： | 斑点酶联免疫吸附试验 兔疥螨 抗体检测 |
Establishment and Application of Dot-ELISA for Detecting Antibodies against Sarcoptes scabiei var.cuniculi |
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| HAO Gui-ying,YANG Guang-you,GU Xiao-bin. Establishment and Application of Dot-ELISA for Detecting Antibodies against Sarcoptes scabiei var.cuniculi[J]. Progress In Veterinary Medicine, 2009, 30(4) |
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| Authors: | HAO Gui-ying YANG Guang-you GU Xiao-bin |
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| Affiliation: | 1.College of Veterinary Medicine;Sichuan Agricultural University;Ya'an;Sichuan;625014;China;2.Department of Animal Science;Xichang College;Xichang;615013;China |
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| Abstract: | The soluble proteins of sarcoptes scabiei var.cuniculi were extracted by repeating freezing thaw,grinding and centrifugation,which was selected as diaphragm-carried antigen in Dot-ELISA.Through repeated experiments,the optimal trial conditions of Dot-ELISA were determined.The specificity was proved by the blocking and cross-reaction tests.The diaphragm did not react with the antibodies against RHDV,E.coli Eperythrozoon and fungi.The diaphragm had the ability to detect the positive serum when it was diluted ... |
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| Keywords: | Dot-ELISA Sarcoptes scabiei var.cuniculi antibody detection |
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