Isolation of Leptospira of the serotype hardjo from bovine kidneys

Thirteen strains of Leptospira serotype hardjo were cultured from 200 bovine kidneys collected from an abattoir. All strains grew on primary isolation in EMJH medium 0.1 per cent Noble agar but most failed to grow in a variety of media containing neomycin or 5-fluorouracil as selective agents. Two of the cattle from which hardjo were isolated were seronegative by the microscopic agglutination test against hardjo, two had titres of 1/100, five of 1/400, three of 1/1600 and one of 1/6400.