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外源生长素对番茄果实蔗糖代谢关键酶活性及基因表达的影响
引用本文:崔娜,李天来,赵聚勇,林凤,白丽萍. 外源生长素对番茄果实蔗糖代谢关键酶活性及基因表达的影响[J]. 华北农学报, 2009, 24(3)
作者姓名:崔娜  李天来  赵聚勇  林凤  白丽萍
作者单位:沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁沈阳,110161;沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁沈阳,110161
基金项目:国家支撑项目,沈阳市科学技术计划项目,沈阳农业大学青年基金项目 
摘    要:以普通栽培型番茄辽园多丽为试验材料,研究了花期施用PCPA对发育过程中的番茄果实糖含量变化、蔗糖代谢关键酶活性及可溶性酸性转化酶基因表达的影响.结果表明,随着番茄果实的发育,可溶性酸性转化酶的活性和酶基因的表达均增强,果糖和葡萄糖的含量也呈递增的趋势,成熟时含量最高.PCPA处理在果实发育的成熟期提高了酸性转化酶的活性,促进了可溶性酸性转化酶基因的表达,增加了果糖和葡萄糖的含量.

关 键 词:番茄  外源生长素  蔗糖代谢  酸性转化酶  基因表达

Effects of Exogenous Auxin on Related Enzymes Activities of Sucrose Metabolism and Gene Expression in Tomato Fruits
CUI Na,LI Tian-lai,ZHA Ju-yong,LIN Feng,BAI Li-ping. Effects of Exogenous Auxin on Related Enzymes Activities of Sucrose Metabolism and Gene Expression in Tomato Fruits[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(3)
Authors:CUI Na  LI Tian-lai  ZHA Ju-yong  LIN Feng  BAI Li-ping
Affiliation:1.College of Horticulture;Key Laboratory of Protected Horticulture of Liaoning Province;Shenyang 110161;China;2.Biological Science and Technology College;Shenyang Agricultural University;China
Abstract:Sugar contents,activities of related enzymes and gene expression of soluble acid invertase during tomato fruit development by PCPA treatment were studied.The results exhibited that activity of acid invertase and gene expression of soluble acid invertase enhanced during fruit development,while the contents of fructose and glucose increased.The activity of acid invertase was improved and gene expression of soluble acid invertase was accelerated during mature period of fruit after PCPA treatment so that the co...
Keywords:Lycopersicon esculentum Mill.  Exogenous auxin  Sucrose metabolism  Acid invertase  Gene expression  
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