Development of sequence characterized DNA markers linked to a temperature dependence for flower induction in lychee (Litchi chinensis Sonn.) cultivars |
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| Affiliation: | 1. Department of Biology Bard College, Annandale-on-Hudson, New York, USA;2. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;3. Institute of Agriculture and forestry, University of Tsukuba, Ibaraki, Japan;1. Escuela Técnica Superior de Ingeniería Agronómica, University of Seville, Carretera de Utrera Km 1, 41013 Sevilla, Spain;2. Unidad Asociada al CSIC de Uso Sostenible del Suelo y el Agua en la Agricultura (US-IRNAS), Crta de Utrera Km 1, 41013 Sevilla, Spain;3. Instituto de Recursos Naturales y Agrobiología (CSIC), P.O. Box 1052, E-41080 Sevilla, Spain;4. Dpto. Riego, Centro de Edafología y Biología Aplicada del Segura (CSIC), P.O. Box 164, E-30100 Espinardo (Murcia), Spain;5. Instituto Nacional de Ciencias Agrícolas, Cuba;6. Unidad Asociada al CSIC de Horticultura Sostenible en Zonas Áridas (UPCT-CEBAS), Paseo Alfonso XIII s/n, E-30203 Cartagena (Murcia), Spain;1. Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan;2. Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan;3. Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277-0882, Japan;1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran;2. Thrombosis Hemostasis Research Center, Tehran University of Medical Sciences, Tehran, Iran;3. Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;1. CIRAD, UMR BGPI, 97130 Capesterre-Belle-Eau, Guadeloupe, France;2. CIRAD, UMR BGPI, F-34398 Montpellier, France;3. CIRAD, UMR PVBMT, 97410 Saint-Pierre, Réunion, France;4. IDIAF, Santo Domingo, Dominican Republic;5. Université des Antilles, LAMIA, 97110 Pointe-à-Pitre, Guadeloupe, France;1. Department of Space and Astronautical Science, The Graduate University for Advanced Studies, 3-1-1 Yoshinodai, Chuo-ku, Sagamihara, Kanagawa 252-5210, Japan;2. School of Physical Sciences, University of Kent, Canterbury CT2 7NH, UK;3. FAM Science Co. Ltd., 6-14-18 Matsumaedai, Moriya, Ibaraki 302-0102, Japan;4. The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 3-1-1 Yoshinodai, Chuo-ku, Sagamihara, Kanagawa 252-5210, Japan;5. Faculty of Medicine, Yamagata University, 2-2-2 Iida-Nishi, Yamagata-shi, Yamagata 990-2331, Japan;6. Planetary Exploration Research Center, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan;7. Institut für Raumfahrtsysteme, Universität Stuttgart, Pfaffenwaldring 29, 70569 Stuttgart, Germany;8. Baylor University, Waco, TX 76706, USA;9. JAXA Space Exploration Center, Japan Aerospace Exploration Agency, 3-1-1 Yoshinodai, Chuo-ku, Sagamihara, Kanagawa 252-5210, Japan |
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| Abstract: | In this paper, we present a method to find DNA markers for traits of interest in lychee cultivars (Litchi chinensis Sonn.) using high-annealing temperature random amplified polymorphic DNA (HAT-RAPD) as an initial screening method. Using 5 arbitrary random primers, a wide range of polymorphic bands ranging from 200 to 5200 bp were produced. Bands of interest were then selected for sequencing and conversion to the more reproducible and robust sequence characterized amplified region (SCAR) markers. Specifically, SCAR markers were found that distinguished lychee varieties requiring a sustained interval at low temperatures for flower induction versus those varieties that do not require such an environment, and another SCAR marker was found that amplified only the economically important Kom cultivar. These sequences shared similarity to known transposons suggesting a mechanism by which the temperature insensitivity may have initially developed. |
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