| 伊犁马在四个微卫星位点上的遗传多样性分析 |
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| 引用本文： | 李红,黄锡霞,姚新奎,苟荣,谭晓海,努尔巴哈提,马江飞,刘珩,玉山江,叶在华. 伊犁马在四个微卫星位点上的遗传多样性分析[J]. 新疆农业科学, 2008, 45(3): 575-578 |
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| 作者姓名： | 李红 黄锡霞 姚新奎 苟荣 谭晓海 努尔巴哈提 马江飞 刘珩 玉山江 叶在华 |
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| 作者单位： | 新疆农业大学动物科学学院,乌鲁木齐,830091;新疆伊犁昭苏马场,新疆昭苏,835600 |
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| 基金项目： | 科技部国际科技合作项目 , 新疆科技攻关项目 |
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| 摘 要： | 通过四个微卫星位点对伊犁马进行了遗传检测 ,用8;非变性聚丙烯酰胺凝胶电泳分离PCR扩增产物.统计该群体的等位基因的组成、等位基因数.利用等位基因频率计算出多态信息含量、杂合度及其平均值.结果表明四个微卫星位点在伊犁马中的平均多态信息含量为0.697 3,属高度多态,可作为有效的遗传标记用于伊犁马遗传多样性的分析.
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| 关 键 词： | 伊犁马 微卫星 遗传多样性 多态信息含量 杂合度 |
| 文章编号： | 1001-4330(2008)03-0575-04 |
| 收稿时间： | 2008-03-25 |
| 修稿时间： | 2008-03-05 |
Analysis on Genetic Diversity at Four Microsatellite Locus of Ili Horse |
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| LI Hong,HUANG Xi-ya,YAO Xin-kui,GOU Rong,TAN Xiao-hai,Nuerbahati,MA Jiang-fei,LIU Heng,Yushanjiang,YE Zai-hua. Analysis on Genetic Diversity at Four Microsatellite Locus of Ili Horse[J]. Xinjiang Agricultural Sciences, 2008, 45(3): 575-578 |
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| Authors: | LI Hong HUANG Xi-ya YAO Xin-kui GOU Rong TAN Xiao-hai Nuerbahati MA Jiang-fei LIU Heng Yushanjiang YE Zai-hua |
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| Abstract: | The genetic polymorphisms of 4 microsatellite were detected in Ili horse.PCR amplification production were separated by 8% nondenaturing PAGE.Number of alleles and alleles frequency were counted.According to alleles frequencies of 4 microsatellite Locus,Polymorphic information contents(PIC),heterozygosity(H)and their mean values were calculatied.The average PIC of 4 microsatellite Locus were 0.697 3,which showed these microsatellite Locus were effective markers for genetic analysis on Ili horse. |
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| Keywords: | Ili horse microsatellite genetic diversity PIC heterpzygosity |
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