不同地理种群二化螟滞育和解除滞育幼虫的抗逆性酶活性比较

测定了不同地理种群二化螟滞育和非滞育幼虫的过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和ATP酶的活性。不同地理种群二化螟滞育幼虫体内5种抗逆性酶的活性没有显著差异;解除滞育幼虫体内的抗逆性酶活力随着纬度的降低而上升,长沙种群的酶活性明显高于哈尔滨种群的酶活性;滞育幼虫体内CAT、POD和SOD酶活性高于解除滞育幼虫体内的酶活性,而ATP和LDH的活性解除滞育幼虫高于滞育幼虫。二化螟滞育幼虫体内酶活力的增高和下降与抗寒有着较密切的关系。不同虫态体内的5种抗逆性酶的活性测定结果显示老熟幼虫体内的CAT、POD和SOD酶活性低于蛹期,而ATP和LDH酶的活性老熟幼虫高于蛹期。

Comparison of the activities of stress-tolerant enzymes in diapaused and diapause-terminated larvae of Chilo suppressalis from different geographic populations

The activities of stress-tolerant enzymes(CAT,POD,SOD,LDH and ATP)were analyzed for diapaused and diapause-terminated Chilo suppressalis(Walker)larvae from different geographic populations.The results showed that the activities of the five enzymes were not significantly different among diapaused larvae from different geographic populations,but increased in diapause-terminated larvae with the decrease of latitude of the geographic populations.The enzyme activities of Changsha population were obviously higher than those of Harbin population.The activities of CAT,POD and SOD were higher in diapaused larvae than in diapause-terminated larvae,while the activities of ATP and LDH were the other way round.Tests of the activities of the five enzymes in mature larvae,prepupae and pupae showed that CAT,POD and SOD were less active in mature larvae than in pupae,while ATP and LDH were more active in mature larvae than in pupae.