| 烟草病源蛋白基因导入马铃薯东农303的研究 |
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| 引用本文： | 李文芙,双宝,李文滨,陈伊里,田兴亚,吕文河,王凤义,朱祥春,秦昕,杨丽娟. 烟草病源蛋白基因导入马铃薯东农303的研究[J]. 东北农业大学学报, 1996, 27(1): 75-80 |
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| 作者姓名： | 李文芙 双宝 李文滨 陈伊里 田兴亚 吕文河 王凤义 朱祥春 秦昕 杨丽娟 |
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| 作者单位： | 东北农业大学生物工程系，东北农业大学农学系 |
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| 摘 要： | 研究建立了一套适于我国北方马铃薯主栽品种东农３０３外源基因导入的体系，并成功地将烟草病原蛋白基因导入该品种．结果显示，块茎外植体比其它外植体更适用于基因导入。农杆菌浓度对Ｔ—ＤＮＡ转移有明显影响，５×１０８ＣＦＵ／ｍＬ是较适宜的浓度．组织化学和分子印迹杂交证明，外源基因已经整合入马铃薯染色体组中。试验选出８个转基因个体，将通过无性系繁殖，进一步鉴定遗传稳定性。
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| 关 键 词： | 马铃薯，外源基因导入，PCR技术 |
INTRODUCTION OF PATHOGENESIS-RELATED PROTEIN GENE INTO POTATO CULTIVAR DONGNONG 303 USING AN AGROBACTERIUM TUMEFACIENS BINARY VECTOR |
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| Li. Wenfu, Shuang Bao, Li Wenbin, Chen yili, Tian Xingya,Wenhe, Wang Fengyi, Zhu Xiungchan, Qin Xin and Yang Lifuan. INTRODUCTION OF PATHOGENESIS-RELATED PROTEIN GENE INTO POTATO CULTIVAR DONGNONG 303 USING AN AGROBACTERIUM TUMEFACIENS BINARY VECTOR[J]. Journal of Northeast Agricultural University, 1996, 27(1): 75-80 |
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| Authors: | Li. Wenfu Shuang Bao Li Wenbin Chen yili Tian Xingya Wenhe Wang Fengyi Zhu Xiungchan Qin Xin Yang Lifuan |
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| Abstract: | In search of establishing a system for genetic tansformation of potato, a pathogenesis-related protein gene from tobacco was successfully transferred into potato cultivar Dong Nong 303 via binary vector. According to southern blot analysis and histochemical assay for-glucuronidase, it was concluded that foreign genes was integrated in potato genome and could be expressed in potato tissues. Of three kinds of explants, a highly efficient introduction was obtained from tuber disc.The concentration of Agrbobacterium tumefaciens had a significent interaction on gene transfer. The most suitable concentration was 5 ×108CFU / mL. From present experiment 8 individuals transferred with foreign gene were selectedand expected to estimate genetic stability using somaclones. |
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| Keywords: | potato introduction of foreign gene PCR |
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