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| 基于线粒体ND4基因的鼢鼠系统进化关系 | |
| 引用本文： | 王海芳,苏军虎,刘荣堂,王静,黄倩. 基于线粒体ND4基因的鼢鼠系统进化关系[J]. 草原与草坪, 2008, 0(4) |
| 作者姓名： | 王海芳 苏军虎 刘荣堂 王静 黄倩 |
| 作者单位： | 1. 甘肃农业大学,生命科学技术学院,甘肃,兰州,730070 2. 甘肃农业大学草业学院,甘肃,兰州,730070 3. 甘肃农业大学,动物科学技术学院,甘肃,兰州,730070 |
| 摘 要： | 为探讨甘肃境内鼢鼠的系统进化关系,采用PCR技术对采集的4种鼢鼠线粒体DNA ND4序列进行扩增,经序列测定后用Clustal X1.83对比,获得了17条长度为425~438 bp不等的同源序列。通过DAN SP4.0比较分析,共检出160个多态信息位点,定义了15种单倍型,单倍型多样度为(Hd):0.980±0.024。采用MEGA4.0中的NJ法、MP法构建的分子进化树表明:高原鼢鼠两群体聚在一起后再与斯氏鼢鼠聚在一起,处于较进化的位置,后与秦岭鼢鼠聚在一起形成一进化枝,再与甘肃鼢鼠群体聚在一起,甘肃鼢鼠最先分化出来,且单独成一进化枝。 |
| 关 键 词： | 鼢鼠 ND4基因 系统进化 |
Systematical evolution enalyzing of four zokors based on Mitochondrial DNA ND4 gene |
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| WANG Hai-fang,SU Jun-Hu,LIU Rong-Tang,WANG Jing,HUANG Qian. Systematical evolution enalyzing of four zokors based on Mitochondrial DNA ND4 gene[J]. Grassland and Turf, 2008, 0(4) | |
| Authors: | WANG Hai-fang SU Jun-Hu LIU Rong-Tang WANG Jing HUANG Qian |
| Affiliation: | 1.College of Life Science andTechnology of Gansu Agricualtural University;Lanzhou 730070;2.Pratacultural Collede of Gansu Agricualtural University;3.Faculty of Animal science and technology of Gansu Agricultural University Lanzhou 730070 |
| Abstract: | To evaluate systematical evolution relationship and provide reasonable basis and reference for classification of Eospala,the ND4 gene of four kinds of zokors were analyzed using PCR method.Direct sequencing 17 individuals of 425～438 bp homologous nucleotide sequences were obtained by using Clustal X1.8,and it showed 160 polymorphic nucleotide sites among homologous sequence and 15 haplotypes were found.Haplotype diversity is 0.980±0.024.In addition,the molecular phylogenetic trees based on ND4 gene was constructed by NJ and MP method using software MEGA4.0,two major evolutionary lineages,Clade A(Myospalax baileyi,M.smith and M.rufescens),and Clade B(M.cansus),were recognized within the specimens.Actually,M.baileyi and M.smith clusters together with high bootstrap values,then clusters together with M.rufescens,while M.cansus remains the basal clade. |
| Keywords: | Eospalax zokors mitochondrial DNA ND4 gene systematical evolutionary |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |