| 用基因枪法将Bt基因转入大豆的研究 |
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| 引用本文： | 王晓春,王罡,季静,王萍. 用基因枪法将Bt基因转入大豆的研究[J]. 大豆科学, 2007, 26(2): 140-143 |
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| 作者姓名： | 王晓春 王罡 季静 王萍 |
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| 作者单位： | 1. 河北北方学院农业科学系,宣化,075131
2. 天津大学农业与生物工程学院,天津,300072 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 以大豆品种合丰25的体细胞胚为受体,以Bt基因为目的基因,应用基因枪法进行了遗传转化,经体细胞胚萌发、生根及壮苗培养和卡那霉素选择培养,获得了完整的抗性再生苗,经PCR、PCR-Southern和点杂交分析鉴定,初步证明外源基因Bt已整合到大豆的基因组中.
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| 关 键 词： | 基因枪 Bt基因 大豆 体细胞胚 遗传转化 |
| 文章编号： | 1000-9841(2007)02-0140-04 |
| 修稿时间： | 2006-11-14 |
PUT BT GENE TRANS FORMATION INTO SMATIC VIA MICROPROJECTILE BOMBARDMENT |
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| WANG Xiao-chun,WANG Gang,JI Jing,WANG Ping. PUT BT GENE TRANS FORMATION INTO SMATIC VIA MICROPROJECTILE BOMBARDMENT[J]. Soybean Science, 2007, 26(2): 140-143 |
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| Authors: | WANG Xiao-chun WANG Gang JI Jing WANG Ping |
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| Abstract: | The Somatic embryo of soybean Hefeng 25 were transformed by microprojectile bombardment.The resistant plants to kanamycin were obtained,two positive plants were obtained among them by PCR,and the positive plantlets were further analyzed by dot blotting and PCR-Southern blotting.The results demonstrated that the target Bt gene had been transformed into the soybean genome. |
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| Keywords: | Soybean Somatic embryos Bt gene Genetic transformation |
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