| 20株土壤粘细菌系统发育研究 |
| |
| 引用本文： | 王芊,张利平.20株土壤粘细菌系统发育研究[J].河北农业大学学报,2010,33(2). |
| |
| 作者姓名： | 王芊 张利平 |
| |
| 作者单位： | 河北大学,生命科学学院,河北省微生物多样性研究与应用实验室,河北,保定,071002 |
| |
| 基金项目： | 基础微生物菌种资源标准化、整理、整合与分享(2005DKA2120605);;河北省应用基础研究计划重点基础研究项目(09966430D) |
| |
| 摘 要： | 对从不同采集地土壤样本中分离的20株粘细菌菌株进行了系统发育分析及G+C mol%测定,以确定其系统发育地位。结果表明:供试菌株分属粘球菌属(Myxococcus)和珊瑚球菌属(Corallococcus)2个属的6个种,其中18株属于粘球菌属(Myxococcus),2株属于珊瑚球菌属(Corallococcus)。各供试菌株与已知相关菌株16SrDNA序列相似性在98.9%~100%之间。各供试菌株G+C mol%在63.12%~73.78%之间,除菌株93357的G+C mol%为63.12%,较其最近相关菌株黄色粘球菌(Myxococcus xanthus)DSM16526低5.26%外,其余同种内菌株的DNA的G+C mol%差异未超过5%,可作为粘细菌16S rDNA序列分析结果的验证性指征。
|
| 关 键 词： | 粘细菌 系统发育分析 G+Cmol% |
Phylogenetic analysis of twenty strains of myxobacteria from soil |
| |
| WANG Qian,ZHANG Li-ping.Phylogenetic analysis of twenty strains of myxobacteria from soil[J].Journal of Agricultural University of Hebei,2010,33(2). |
| |
| Authors: | WANG Qian ZHANG Li-ping |
| |
| Institution: | Key Laboratory of Microbial Diversity Research and Application of Hebei Province;College of Life Sciences;Hebei Uniersity;Baoding 071002;China |
| |
| Abstract: | 20 myxobacteria strains isolated from different soil samples were studied by phylogenetic analysis and determination of genomic DNA G+C content.The 16S rRNA gene sequence analysis showed that the sequence similarities of the test strains and related strains are between 98.9% and 100%,which indicated that 18 strains belong to Myxococcus and 2 strains to Corallococcus.The genomic DNA G+C content of 20 strains range from 63.12% to 73.78%.Meanwhile,by comparing the genomic DNA G+C content of the test strains an... |
| |
| Keywords: | myxobacteria phylogenetic analysis G+C mol% |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
