铁皮石斛试管苗快繁体系

铁皮石斛Dendrobium officinale是一种名贵药用植物,野生资源亟待保护,必须通过试管苗快繁技术研究,才能满足人们对它的需要。通过不同基本培养基对铁皮石斛进行种子发芽试验,不同消毒方法对茎尖进行存活率试验,不同植物生长调节物质组合对不定芽、原球茎和壮苗及根系的诱导试验的结果表明:铁皮石斛胚培养最佳培养基为1 2 MS(Murashige and Skoog)+添加物A+ 25 gL- 1蔗糖; 4种消毒方法中最佳消毒方法为B,外植物茎尖存活率可达76.2%。不定芽诱导最佳培养基为1 2MS+ 1.5 mgL- 1 6-苄基腺嘌呤(BA)+ 0.25 mgL- 1萘乙酸(NAA),诱导倍数4.7。原球茎诱导最佳培养基为1 2 MS+ 1.0 mgL- 1 BA+ 0.5mgL- 1 NAA,诱导率为58%。不定芽最佳生根培养基为1 2MS+ 0.5 mgL- 1 NAA,生根率为100%,平均生根数为7.1条。建立了铁皮石斛试管苗快繁体系,为其广泛应用提供了依据。图1表5参11

Micropropagation of Dendrobium officinale

Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch, 1 fig. 5 tab. 11ref.]