| 月季花粉离体萌发液体培养基组分的优化 |
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| 引用本文： | 王岚岚,游捷,俞红强.月季花粉离体萌发液体培养基组分的优化[J].河北农业大学学报,2008,31(3). |
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| 作者姓名： | 王岚岚 游捷 俞红强 |
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| 作者单位： | 中国农业大学农学与生物技术学院,北京,100094 |
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| 摘 要： | 以月季品种‘Sorbet fruite'的花粉为材料,通过单因子试验比较了液体培养基中蔗糖及B、Ca、Mg、K对月季花粉离体萌发的影响,以此为基础利用L_(25)(5~6)的正交试验,比较了蔗糖、Ca(NO_3)_2·4H_2O、H_3BO_3对月季花粉萌发的影响。结果表明:蔗糖、Ca(NO_3)_2·4 H_2O和蔗糖×H_3BO_3的交互作用对‘Sorbet fruite'花粉萌发有极显著影响。适宜培养液为蔗糖20%+H_3BO_3(50～100)mg/L+Ca(NO_3)_2·4 H_2O(100～200)mg/L。在适宜的培养液中加入30%的PEG-4000,萌发率和花粉管长度均有极显著增加。
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| 关 键 词： | 月季 花粉 液体培养基 离体萌发 |
The optimization of culture solution for rose pollen in vitro germination |
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| WANG Lan-lan,YOU Jie,YU Hong-qiang.The optimization of culture solution for rose pollen in vitro germination[J].Journal of Agricultural University of Hebei,2008,31(3). |
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| Authors: | WANG Lan-lan YOU Jie YU Hong-qiang |
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| Abstract: | Fresh pollen of‘Sorbet fruite'was employed as experimental material,and comparisons were made for the effects of sucrose,boron,calcium,magnesium and potassium on the in vitro ger- mination of rose pollen through simple factorial experiment.And L_(25)(5~6)orthogonal design was used to compare the effects of sucrose,boron,calcium on the germination of rose pollens.Results showed that sucrose,calcium and sucrose×boron interaction have great effects on the in vitro ger- mination of rose pollen.The optimum culture solution was sucrose 20%+H_3BO_3(50-100)mg/L+ Ca(NO_3)_2·10H_2O(100-200)mg/L.The optimal culture solution supplemented with 40 % PEG- 4000 increased both the pollen germination rate and the pollen tube length significantly. |
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| Keywords: | rose pollen culture solution in vitro germination |
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