| 水稻草状矮化病毒沙县分离株基因组第六片段的序列分析 |
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| 引用本文： | 张春嵋,吴祖建,林丽明,林奇英,谢联辉.水稻草状矮化病毒沙县分离株基因组第六片段的序列分析[J].植物病理学报,2001,31(4):301-305. |
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| 作者姓名： | 张春嵋 吴祖建 林丽明 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所, 福建省植物病毒学重点实验室, 福州 350002 |
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| 基金项目： | 福建省科委重点项目(95-Z-4) |
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| 摘 要： | 根据已报道的水稻草状矮化病毒(Rice grassy stunt virus,RGSV) IRRI分离株第6片段的RNA序列设计引物,通过RT-PCR得到覆盖RGSV沙县分离株(RGSV-SX)基因组第6片段全长序列的cDNA克隆并进行序列分析,发现其与IRRI分离株的核苷酸序列同源性高达99.3%,并对这一结果的可能原因作出分析。
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| 关 键 词： | 水稻草矮病毒 RNA6 序列分析 |
| 文章编号： | 0412-0914(2001)04-0301-05 |
| 收稿时间： | 1999-09-22 |
| 修稿时间： | 1999年9月22日 |
SEQUENCE ANALYSIS OF RNA6 OF THE RGSV ISOLATE FROM SHAXIAN |
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| ZHANG Chun mei,WU Zu jian,LIN Li ming,LIN Qi ying,XIE Lian hui.SEQUENCE ANALYSIS OF RNA6 OF THE RGSV ISOLATE FROM SHAXIAN[J].Acta Phytopathologica Sinica,2001,31(4):301-305. |
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| Authors: | ZHANG Chun mei WU Zu jian LIN Li ming LIN Qi ying XIE Lian hui |
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| Institution: | Institute of Plant Virology, Fujian Agriculture and Forestry University, Fujian Provincial Key Laboratory of Plant Virology, Fuzhou 350002, China |
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| Abstract: | Specific primers were designed according to the sequence of a Rice grassy stunt virus (RGSV) isolate from IRRI. By RT PCR with these primers, three DNA fragments covering the full length of RNA segment 6 of the RGSV isolate from Shaxian were obtained, and subsequently sequenced. Results of this study showed that the identity between the sixth RNA fragment of these two isolates was as high as 99.3%. |
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| Keywords: | Rice grassy stunt virus RNA6 sequence analysis |
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