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| 高效阴离子交换色谱法测定毛头鬼伞多糖中的单糖组成 | |
| 引用本文： | 杨仁智,张劲松,唐庆九,潘迎捷.高效阴离子交换色谱法测定毛头鬼伞多糖中的单糖组成[J].中国食用菌,2005,24(5):42-44. |
| 作者姓名： | 杨仁智 张劲松 唐庆九 潘迎捷 |
| 作者单位： | 1. 南京农业大学微生物学系,南京,210095 2. 上海市农科院食用菌研究所,上海,201106 3. 上海水产大学,上海,200090 |
| 基金项目： | 上海市农委重点攻关项目（No.沪农科攻字2003-3-3） |
| 摘 要： | 本文采用高效阴离子交换色谱—脉冲安培检测器（HAPEC-PAD），建立了一种测定多糖中单糖比例的方法。以NaOH为淋洗液、CarboPac^TM A20预处理柱，CarboPac^TMPA20分离柱，金工作电极，Ag/AgCl参比电极，8种自然界中常见单糖标准品做混合标样，探索方法可行性。在淋洗液浓度为2．5mmol／L时，各种单糖组分得到有效分离，其线性和重现性均良好并在此基础上测定了毛头鬼伞多糖中单糖比例；与传统的方法相比，此方法具有前处理简单、灵敏度高、节省时间和试剂等优点。 |
| 关 键 词： | 离子交换色谱 多糖 单糖比例 |
| 文章编号： | 1003-8310(2005)05-0042-03 |
High Performance Anion Exchange Chromatography Method to Determine the Monosaccharide Composition of Polysaccharides |
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| YANG Ren-zhi,ZHANG Jin-song,TANG Qin-jiu,PAN Ying-jie.High Performance Anion Exchange Chromatography Method to Determine the Monosaccharide Composition of Polysaccharides[J].Edible Fungi of China,2005,24(5):42-44. | |
| Authors: | YANG Ren-zhi ZHANG Jin-song TANG Qin-jiu PAN Ying-jie |
| Abstract: | This paper offers a method to determine the monosaccharides'composition by high performance anion exchange chromatography-pulsed ampere detector(HPAEC-PAD).With NaOH eluent,a gold work electrode and a Ag/AgCl reference electrode,CarboPac~(20) PA20 guard column,CarboPac~(TM) PA20 analytical column and 8 monosaccharides as a mixed standard the feasibility is studied.The result shows that at 2.5 mmol/L NaOH each monosaccharide can be well separated;Their linear relationship and repeatability are good.In the same condition,the monosaccharides'composition of the polysaccharides from the fruiting bodies of coprinus comatus was determined.This method is better than traditional methods because it does not need derivation reaction and detects lower concentration and saves time and reagents. |
| Keywords: | High performance anion exchange chromatography Polysaccharides Monosaccharide composition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |