番茄抗晚疫病材料的鉴定及初步转育

 对引入的LA1033、LA2099、LA1777等3份多毛番茄人工接种晚疫病, 证明其均高抗我国番茄晚疫病生理小种T10203, 其中LA2099和LA1777还高抗致病力极强的小种T1020304。直播杂交转育F₁种子可出苗, 但出苗率极低, 且不同母本遗传背景和多毛番茄材料组合间差异明显。与直播相比, 普通MS培养基培养成熟胚可更快速、有效地获得F₁ 杂交种植株。人工辅助授粉F₁ 自交, 田间坐果数也存在明显不同。

Identification of Tomato Wild Species Resistant to Late Blight(Phytophthora infestans) and Primary Introgression into Cultivars in China

The results from seedling inoculation on 3 accessions Lycopersicon hirsutum including LA1033, LA2099 and LA1777 proved that all of them gave a high resistance to Phytophthora infestans strains T1,2,3. Moreover, LA2099 and LA1777 also produced a high resistance to strain T1, 2, 3,4, which was much more pathogenicity for tomato in China. F1 seeds from crossing these species with tomato lines were sowed in the greenhouse but gave very low germination rate. Significant difference was observed among different combinations, and it mainly depends on the different maternal genetic background and wild accessions. Comparing to direct sowing, mature embryo rescue in MS medium could provide more convenient, efficient and easy way to get F1 plants. Fruit setting of F1 pollinated by artificial pollination in the field indicated the great difference which was existed among the combinations. The above identified wild species and the introgression progenies would provide the potential use for tomato breeding resistant to Phytophthora infestans in China.