miR-222-3p在鹅肥肝形成中的作用及靶基因验证

为了检测miR-222-3p在填饲鹅不同组织中的表达情况，并对其靶基因进行预测和验证，探讨miR-222-3p在鹅肥肝形成中的功能。运用实时荧光定量 PCR技术检测miR-222-3p 在填饲鹅肝脏、胸肌和腹脂中的表达量；采用生物信息学方法对鹅miR-222-3p的靶基因进行预测，荧光定量PCR技术检测预测靶基因在肝脏中的表达量，并利用双荧光素酶基因报告系统验证其靶向关系。结果显示：相比对照组，miR-222-3p 在填饲鹅肝脏和腹脂中的表达量均显著升高；生物信息学预测结果显示MARF1和B4GALNT3基因在3’UTR区域存在miR-222-3p的潜在结合位点，且这两个基因在鹅肥肝中均显著下调，而双荧光素酶基因报告系统显示只有MARF1基因与鹅 miR-222-3p存在靶向关系。结果表明，miR-222-3p在鹅肥肝中表达量显著上调，且可能通过其靶基因MARF1对鹅肥肝的形成发挥调控作用。

Role of miR-222-3p in goose fatty liver formation and validation of target genes

In order to detect the expression of microRNA-222-3p in different tissues of feeding geese to predict and validate the target genes of microRNA-222-3p and to explore the function of microRNA-222-3p in the formation of goose fatty liver. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-222-3p in the liver, pectoral muscle and abdominal fat of goose, bioinformatics was used to predict the target gene of microRNA-222-3p, quantitative fluorescence PCR was used to detect the predicted target gene expression in liver, and double luciferase gene reporting system was used to verify the target gene. The results showed that the expression of microRNA-222-3p was significantly increased in both liver and abdominal fat of feeding geese compared with the control group, the expression of bioinformatics predictions showed that MARF1 and B4GALNT3 had potential binding sites of microRNA-222-3p in the 3''UTR region, and both genes were significantly down-regulated in the fatty liver of geese. However, the dual-luciferase gene reporting system showed that only MARF1 was the target gene of microRNA-222-3p in geese. The results indicated that microRNA-222-3p expression was significantly up-regulated in goose fatty liver, and may play a regulatory role in the formation of goose fatty liver through its target gene MARF1.