Chromosome doubling through adventitious shoot formation on in vitro cultivated leaf explants from diploid interspecific potato hybrids |
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| Authors: | J. G. Th. Hermsen M. S. Ramanna S. Roest G. S. Bokelmann |
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| Affiliation: | (1) Institute of Plant Breeding (IvP), Agricultural University, Wageningen, the Netherlands;(2) Foundation ITAL., Wageningen, the Netherlands |
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| Abstract: | Summary Nearly 450 plantlets were produced from 51 diploid Solanum etuberosum x S. pinnatisectum F1 hybrids through adventitious shoot formation on in vitro cultivated rachis and petiole explants.On the basis of phenotypical assessment of the ploidy level of 425 plants, 84.7% of the plants were scored as doubled or doubled twice. A cytological analysis of ploidy in the three layers L1.L2 and L3 of 112 plants revealed 83.9% complete doubling: periclinal ploidy chimeras were not found and only two sectorial ploidy chimeras were detected. Doubled plants were obtained from all 51 clones.Various flower colours and epinastic leaves (in 1 clone) may be indications of mutagenesis through the treatment. |
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| Keywords: | Solanum potato explant culture interspecific hybrid adventitious shoots in vitro chromosome doubling solid mutants |
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