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Assembly of chick brain tubulin onto isolated basal bodies of Chlamydomonas reinhardi
Authors:W J Snell  W L Dentler  L T Haimo  L I Binder  J L Rosenbaum
Abstract:Basal bodies isolated from Chlamydomonas reinhardi will serve as initiation centers for the assembly of chick brain microtubule protein subunits (tubulin) into microtubules. The rate of microtubule assembly is tubulin-concentration dependent; this assembly occurs onto both distal and proximal ends of the basal body mnicrotubules, with distal assembly greatly favored. In vitro assembly of brain tubulin also occurs onto the mid-lateral aspects of the basal bodies, presumably onto the fiber connecting the two basal bodies.
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