| 鹅副粘病毒HN基因在昆虫杆状系统表达及其抗原性鉴定 |
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| 引用本文： | 藏玉婷,周丽,姜亦飞,王君伟.鹅副粘病毒HN基因在昆虫杆状系统表达及其抗原性鉴定[J].东北农业大学学报,2008,39(3):88-91. |
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| 作者姓名： | 藏玉婷 周丽 姜亦飞 王君伟 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030 |
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| 摘 要： | 血凝素-神经氨酸酶蛋白(HN)是鹅副粘病毒诱导体液免疫的重要靶抗原。经pMD18-T-HN阳性质粒扩增了HN基因,亚克隆至昆虫杆状病毒转移载体pBlueBacHis2A,并将其与线性化的杆状病毒共转染对数生长期的sf 9细胞,得到重组杆状病毒rBv-HN,试验表明,HN在昆虫细胞中得到表达,且产物具有抗原性。
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| 关 键 词： | 鹅副粘病毒 HN基因 昆虫杆状系统表达 抗原性 |
| 文章编号： | 1005-9369(2008)03-0088-04 |
| 修稿时间： | 2007年3月22日 |
Recombinant baculovirus expression of HN gene of GPMV and the antigenicity of the expressed protein |
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| ZANG Yuting,ZHOU Li,JIANG Yifei,WANG Junwei.Recombinant baculovirus expression of HN gene of GPMV and the antigenicity of the expressed protein[J].Journal of Northeast Agricultural University,2008,39(3):88-91. |
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| Authors: | ZANG Yuting ZHOU Li JIANG Yifei WANG Junwei |
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| Abstract: | Haemagglutinin Neuramindase(HN) protein is the important antigen of goose paramyxovirus which can induce humoral immunity.Using a pair of specific primers designed according to the pMD18-T-HN,the HN gene of goose paramyxovirus was amplified by PCR.The PCR product was cloned into pBlueBacHis2A,and then sf9 cells were transfected with the recombinant plasmid.Recombinant baculovirus rBv-HN was obtained.West-ernBlotting showed HN is well expressed,and its product have antigenicity. |
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| Keywords: | goose paramyxovirus HN gene baculoviruse expression antigenicity |
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