| 响应面法研究鱼鳞胃蛋白酶酶解特性 |
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| 引用本文： | 周颖,林志峰,吴琼,于晓菊,刘石生,易美华.响应面法研究鱼鳞胃蛋白酶酶解特性[J].农村实用技术与信息,2009(7):18-20. |
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| 作者姓名： | 周颖 林志峰 吴琼 于晓菊 刘石生 易美华 |
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| 作者单位： | 海南大学食品学院,热带生物资源教育部重点实验室,海南海口570228 |
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| 基金项目： | 海南省教育厅指导性项目（Hj200603）,国家科技支撑计划项目（2007BAD6806）,国家科技支撑计划项目（2007BAD29809） |
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| 摘 要： | 以响应面试验设计方法研究了胃蛋白酶对鱼鳞蛋白的水解特性．得到拟合良好的响应面方程，在选定范围内胃蛋白酶水解鱼鳞蛋白的最优条件为：pH=1．5，时间为6h，温度为60℃，此时蛋白得率的最优值为164．0mg／g原料。
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| 关 键 词： | 响应面 鱼鳞蛋白 胃蛋白酶 |
Study on Hydrolysis of Fish Scale Protein by Pepsin with Responsesurfaee Method |
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| Institution: | ZHOU Ying, LING Zhi- feng, WU Qiong, YU Xiao-ju, LIU Shi- sheng, YI Mei- hua ( The Key Laboratory of Tropical Biological Resources Ministry of Education, Food College, Hainan University, Haikou, Hainan 570228, China) |
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| Abstract: | The hydrolysis characters of pepsin on fish scale protein was studied with responsesurfaee method, which leads to a fitting equation between protein extract rate and pH, time, temperature. The results shows that the optimized conditions for fish scale protein hydrolysis by pepsin is as follows： pill. 5, 6 hours, 60℃. Under the optimized conditions, the best extract rate is 164.0 mg/ g scale. |
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| Keywords: | responsesurfaee fish scale protein pepsin |
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