浅黄根须腹菌的分离及培养特性研究

研究以油松外生茵根真菌浅黄根须腹茵（Rhizopogonluteolus）的子实体作为分离材料，通过采用组织分离方法获得纯菌种。以菌丝体生长速度和生长状况为参照标准，将菌种接种于不同培养基，筛选出茵丝体生长最适基础培养基为PACH，在此基础上通过单因素试验对其pH值，碳源，氮源等培养条件进行研究，结果表明，菌丝体生长最佳pH值，碳源及氮源分别为6．0、葡萄糖和酒石酸铵。通过pH值、碳源及氮源3个较优水平的正交试验，确定在（pH5．0＋牛肉膏＋蔗糖）组合中茵丝体生长速度最快，而在（pH6．O＋牛肉膏＋葡萄糖）组合中茵丝体生长状况为最佳。

Study on the Isolation and Cultural Characteristics of Rhizopogon luteolus

In this study, the fruiting body of Rhizopogon luteolus that is ecto-mycorrhizal fungi of Pinus tabulaeformis was considered as a separation material and the pure cultures of Rhizopogon luteolus were obtained by tissue culture. Strain inoculated in different culture medium with a reference standard of mycelium growth rate and growth situation. The basic culture medium PACH was selected as a suitable growth of mycelium. Based on this, the cultural characteristics like pH, carbon source, nitrogen source were studied by single factor tests. The research results showed that the pH6.0, glucose, Ammonium tartrate were the best pH value, carbon and nitrogen source respectively. And the growth of mycelium was the fastest in （pHS.0＋beef extract＋ sucrose） combination, the growth situation of mycelium was the best in （pH6.0＋beef extract＋ glucose） combination. By the three optimum level of pH, carbon and nitrogen sources orthogonal tests.