| 类黄酮3',5'羟基化酶基因的克隆及转化铁炮百合 |
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| 引用本文： | 徐碧玉,刘菊华,金志强.类黄酮3',5'羟基化酶基因的克隆及转化铁炮百合[J].园艺学报,2005,32(6):1051-1055. |
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| 作者姓名： | 徐碧玉 刘菊华 金志强 |
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| 作者单位： | (中国热带农业科学院热带生物技术研究所, 热带作物生物技术国家重点实验室, 海口571101) |
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| 基金项目： | 中国热带农业科学院重点基金资助项目 |
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| 摘 要： | 利用RT-PCR技术从矮牵牛的紫色花瓣中克隆了两个控制花色的类黄酮3',5'羟基化酶基因Hf 1和Hf 2, 并构建了植物表达载体, 利用农杆菌介导法对铁炮百合进行了遗传转化, 获得了转基因植株。
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| 关 键 词： | 铁炮百合 类黄酮3' 5'羟基化酶基因 转化 |
| 文章编号： | 0513-353X（2005）06-1051-05 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-04-202005-10-26 |
Cloning of Flavonoid-3',5'-hydroxylase Gene and Its Transformation into Lily (Lilium longiforum) |
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| Xu Biyu,Liu Juhua,Jin Zhiqiang.Cloning of Flavonoid-3'''',5''''-hydroxylase Gene and Its Transformation into Lily (Lilium longiforum)[J].Acta Horticulturae Sinica,2005,32(6):1051-1055. |
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| Authors: | Xu Biyu Liu Juhua Jin Zhiqiang |
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| Institution: | 1.West China Hospital, Sichuan University，2College of basic Medicine and Forenscic Medical Science，3West China Second Hospital, Sichuan University, Chengdu 610041, China |
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| Abstract: | Flavonoid-3',5'-hydroxylase(F3',5'H) is a key enzyme in the biosynthesis of anthocyanins,which can divert the production of delphinidin pigments.Two cDNAs designated as Hf1 and Hf2 encoding F3',5'H were isolated from the purple flower of Petunia hybrida by using RT-PCR and also sequenced.A construct of Hf 2 in a sense-orientation driven by CaMV 35S promoter was constructed and transformed into Lily(Lilium longiforum) by using Agrobacterium tumefacien mediated method.The transgenic plants were confirmed that Hf 2 gene was integrated into lily genome by PCR and Southern blot. |
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| Keywords: | Lilium longiflorum Flavonoid-3' 5'-hydroxylase Genetic transformation |
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