Stimulation by phytohaemagglutinin of peripheral blood lymphocytes from horse,pig, sheep and man |
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| Authors: | GJ Peters JH Veerkamp |
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| Institution: | Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen The Netherlands |
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| Abstract: | Optimal conditions for stimulation by phytohaemagglutinin (PHA) were established for equine, porcine, ovine and human lymphocytes in MEMS medium. Optimal thymidine concentration was determined for assay of cell transformation. With all species tested horse serum gave highest thymidine incorporation. Homologous serum was not more appropriate for lymphocytes of man, pig and sheep. Optimal stimulation was achieved at 20, 0.5–5, 5, and 10–40 μg PHA per 106 cells for human, equine, porcine and ovine lymphocytes, respectively. |
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