| 胞外DNA在土壤中的固定与转化 |
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| 引用本文： | CAI Peng,HUANG Qiao-Yun,ZHANG Xue-Wen,CHEN Hao. 胞外DNA在土壤中的固定与转化[J]. 土壤圈, 2005, 15(1): 16-23 |
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| 作者姓名： | CAI Peng HUANG Qiao-Yun ZHANG Xue-Wen CHEN Hao |
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| 作者单位： | [1]StateKeyLaboratoryofAgriculturalMicrobiology,CollegeofResourcesandEnvironment,HuazhongAgricultureUniversity,Wuhan430070China [2]StateKeyLaboratoryofAgriculturalMicrobiology,CollegeofResourcesandEnvironment,HuazhongAgricultureUniversity,Wuhan430070China／／CollegeofScience,HuazhongAgricultureUniversity,Wuhan430070China [3]CollegeofScience,HuazhongAgricultureUniversity,Wuhan430070China |
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| 基金项目： | *1Project supported by the National Natural Science Foundation of China (No.40271064). |
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| 摘 要： | DNA is the genetic material of various organisms. Extracellular DNA adsorbed or bound on surface-active particles in soils has been shown to persist for long periods against nucleases degradation and still retain the ability to transform competent cells. This paper reviews some recent advances on the binding and transformation of extracellular DNA in soils,which is fundamental to understanding the nature of the soil, regulating biodiversity, and assessing the risk of releasing genetically engineered microorganisms (GEMs) as well as being helpful for development of the genetic evolutional theory of bacteria. Several influencing factors, such as soil pH, ionic strength, soil surface properties, and characteristics of the DNA polymer, are discussed. To date, the understanding of the type of molecular binding sites and the conformation of adsorbed and bound DNA to soil particles is still in its infancy.
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| 关 键 词： | 土壤生态学 DNA 脱氧核糖核酸 基因材料 组织吸收 |
Binding and transformation of extracellular DNA in soil |
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| CAI Peng,HUANG Qiao-Yun,ZHANG Xue-Wen and CHEN Hao. Binding and transformation of extracellular DNA in soil[J]. Pedosphere, 2005, 15(1): 16-23 |
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| Authors: | CAI Peng HUANG Qiao-Yun ZHANG Xue-Wen CHEN Hao |
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| Affiliation: | State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agriculture University, Wuhan 430070 (China). E-mail: cpcp@webmail.hzau.edu.cn;State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agriculture University, Wuhan 430070 (China). E-mail: cpcp@webmail.hzau.edu.cn;State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agriculture University, Wuhan 430070 (China). E-mail: cpcp@webmail.hzau.edu.cn;College of Science, Huazhong Agriculture University, Wuhan 430070 (China) |
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| Abstract: | DNA is the genetic material of various organisms. Extracellular DNA adsorbed or bound on surface-active particles in soils has been shown to persist for long periods against nucleases degradation and still retain the ability to transform competent cells. This paper reviews some recent advances on the binding and transformation of extracellular DNA in soils,which is fundamental to understanding the nature of the soil, regulating biodiversity, and assessing the risk of releasing genetically engineered microorganisms (GEMs) as well as being helpful for development of the genetic evolutional theory of bacteria. Several influencing factors, such as soil pH, ionic strength, soil surface properties, and characteristics of the DNA polymer, are discussed. To date, the understanding of the type of molecular binding sites and the conformation of adsorbed and bound DNA to soil particles is still in its infancy. |
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| Keywords: | adsorption binding extracellular DNA soil transformation |
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