NaCl处理对酸枣幼苗内源激素含量的影响

【目的】研究不同浓度NaCl处理对酸枣幼苗叶和根系内源激素含量的影响。【方法】利用高效液相色谱法,测定不同NaCl浓度处理及不同时间下,酸枣幼苗叶、根中脱落酸、生长素、赤霉素、玉米素4种内源激素的含量。【结果】与0 mmol/L NaCl处理相比,处理时间相同时,酸枣幼苗叶和根在50、100、150、200 mmol/L NaCl处理下ABA含量显著增加,IAA、ZT、GA₃的含量、IAA/ABA、GA₃/ABA的比值整体显著下降。与处理0 h相比,不同浓度NaCl处理下,随着处理时间的延长,酸枣叶,根的ABA含量显著升高,IAA/ABA、GA₃/ABA的比值均显著降低;100 mmol/L NaCl处理下,酸枣叶和根的GA₃、IAA、ZT含量显著下降。【结论】NaCl处理下,处理时间相同时,酸枣幼苗通过增加叶和根ABA含量,降低叶和根IAA、ZT、GA₃含量、IAA/ABA和GA₃/ABA的比值适应盐胁迫。

The Change of Endogenous Hormone Content in Zizyphus Jujube Seedlings Treated by NaCl

【Objective】 To investigate the effects of different concentrations of NaCl on endogenous hormones in leaves and roots of jujube seedlings. 【Method】HPLC method was applied to determine the contents of abscisic acid, auxin, gibberellin and zeatin in leaves and roots of Zizyphus jujube seedlings treated by NaCl with different concentrations and various treating times.【Result】Compared with 0 mmol/L NaCl treatment, ABA content in leaves and roots of Zizyphus jujube seedlings treated for the same time increased significantly under 50, 100, 150, 200 mmol/L NaCl treatment, contents of IAA, ZT, GA₃ as well as the ratio of IAA/ABA, GA₃/ABA decreased remarkably. Compared with 0 h treatment, the ABA content in leaves and roots of Zizyphus jujube treated by NaCl with different concentrations increased noticeably with the increase of treating time, and ratios of IAA/ABA, GA₃/ABA decreased dramatically. Under 100 mmol/L NaCl treatment, contents of GA₃, IAA and ZT in leaves and roots of Zizyphus jujube decreased obviously.【Conclusion】Under NaCl treatment, when the treating time was the same, Zizyphus jujube seedling adapted to salt stress by increasing ABA content while decreasing contents of IAA, ZT, GA₃ and ratios of IAA/ABA, GA₃/ABA in its leaves and roots.